KMID : 0921620100400020067
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Journal of Bacteriology and Virology 2010 Volume.40 No. 2 p.67 ~ p.75
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Analysis of Low Molecular Weight Proteome from H. pylori Cell Extract Using the High Performance Liquid Chromatography
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Park Jung-Won
Lee Kyung-Ja Kim Kyung-Mi Joo Jung-Soo Kwon Yung-Chul Youn Hee-Shang Song Jae-Young Kang Hyung-Lyun Lee Kon-Ho Baik Seung-Chul Lee Woo-Kon Cho Myung-Je Rhee Kwang-Ho
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Abstract
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Low molecular proteins (LMPs) which are smaller than 20 kDa are difficult to visible on a standard two-dimensional SDS-polyacrylamide gel electrophoresis (2-D SDS-PAGE) map. LMPs must be enriched appropriately to be analyzed. We isolated LMPs of Helicobacter pylori 26695 from 1-D polyacrylamide gel and digested by pepsin. Pepsin-digested LMPs were separated by HPLC and each fraction was analyzed by hybrid tandem mass spectrometer. Seventy nine peptides, representing 27 genes, including copper ion binding protein (CopP, 7 kDa), thioredoxin (TrxA, 11.9 kDa) and ribosomal protein L23 (Rpl23, 10.5 kDa) were identified. Some proteins larger than 40 kDa including Omp2, Omp21, Omp27, Omp30, Omp32, catalase and HP1083 were also identified. This work may give researchers a useful way to analyse the expressed LMPs which could not be identified on the conventional 2-D SDS-PAGE.
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KEYWORD
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Helicobacter pylori, Hybrid tandem mass spectrometer, Low molecular protein
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